Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24333335
DOI
10.1016/j.febslet.2013.11.038
PII: S0014-5793(13)00885-5
Knihovny.cz E-zdroje
- Klíčová slova
- 1,6-diphenyl-1,3,5-hexatriene, DHS, DL-dihydrosphingosine, DPH, Fluidity, Fluorescence anisotropy, Lipid order, Membrane microdomain, Time-resolved fluorescence, all-trans-9,11,13,15-octadecatetraenoic acid, t-PnA,
- MeSH
- buněčná membrána chemie metabolismus MeSH
- difenylhexatrien chemie MeSH
- fluorescenční polarizace MeSH
- fluorescenční spektrometrie MeSH
- membránové mikrodomény chemie metabolismus MeSH
- mutace MeSH
- nenasycené mastné kyseliny chemie MeSH
- Saccharomyces cerevisiae MeSH
- sfingolipidy biosyntéza chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- difenylhexatrien MeSH
- nenasycené mastné kyseliny MeSH
- parinaric acid MeSH Prohlížeč
- sfingolipidy MeSH
We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.
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