Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24491788
DOI
10.1016/j.aca.2013.12.032
PII: S0003-2670(13)01581-X
Knihovny.cz E-resources
- Keywords
- Biofilm, Candida parapsilosis group, Capillary isoelectric focusing, High-performance liquid chromatography, Micropreparative solution isoelectric focusing, Sonicate from vascular catheter,
- MeSH
- Biofilms * MeSH
- Candida isolation & purification MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Isoelectric Focusing methods MeSH
- Hydrogen-Ion Concentration MeSH
- Humans MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Catheters, Indwelling microbiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material - sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.
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