Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24530738
DOI
10.1016/j.lfs.2014.01.082
PII: S0024-3205(14)00220-3
Knihovny.cz E-resources
- Keywords
- H(2)R, H(4)R, Histamine, Histamine receptors, Phagocytes, Reactive oxygen species,
- MeSH
- Histamine Agonists pharmacology MeSH
- Benzimidazoles pharmacology MeSH
- Dimaprit pharmacology MeSH
- Phagocytes drug effects metabolism MeSH
- Guanidines pharmacology MeSH
- Histamine physiology MeSH
- Receptors, Histamine H4 MeSH
- Humans MeSH
- Methylhistamines pharmacology MeSH
- Reactive Oxygen Species blood MeSH
- Receptors, Histamine H2 metabolism MeSH
- Receptors, Histamine metabolism MeSH
- Receptors, G-Protein-Coupled agonists metabolism MeSH
- Thiourea analogs & derivatives pharmacology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4-methylhistamine MeSH Browser
- Histamine Agonists MeSH
- Benzimidazoles MeSH
- Dimaprit MeSH
- Guanidines MeSH
- Histamine MeSH
- Receptors, Histamine H4 MeSH
- HRH4 protein, human MeSH Browser
- JNJ 10191584 MeSH Browser
- Methylhistamines MeSH
- Reactive Oxygen Species MeSH
- Receptors, Histamine H2 MeSH
- Receptors, Histamine MeSH
- Receptors, G-Protein-Coupled MeSH
- S-(2-guanidylethyl)isothiourea MeSH Browser
- Thiourea MeSH
AIMS: The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. MAIN METHODS: Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol-HRP-H2O2 based CL). KEY FINDINGS: Histamine, 4-methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP-activated whole blood CL in a dose-dependent manner. On the other hand, only VUF8430 was able to inhibit PMA-activated whole blood CL. Ranitidine, but not JNJ10191584, completely reduced the effects of histamine, 4-methylhistamine and dimaprit. The direct scavenging ability of tested compounds was negligible. SIGNIFICANCE: Our results demonstrate that the inhibitory effects of histamine on ROS production in whole blood phagocytes were caused by H2R. Our results also suggest that H4R agonists in concentrations higher than 10(-6)M may also influence ROS production via binding to H2R.
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