Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24530738
DOI
10.1016/j.lfs.2014.01.082
PII: S0024-3205(14)00220-3
Knihovny.cz E-zdroje
- Klíčová slova
- H(2)R, H(4)R, Histamine, Histamine receptors, Phagocytes, Reactive oxygen species,
- MeSH
- agonisté histaminu farmakologie MeSH
- benzimidazoly farmakologie MeSH
- dimaprit farmakologie MeSH
- fagocyty účinky léků metabolismus MeSH
- guanidiny farmakologie MeSH
- histamin fyziologie MeSH
- histaminový receptor H4 MeSH
- lidé MeSH
- methylhistaminy farmakologie MeSH
- reaktivní formy kyslíku krev MeSH
- receptory histaminu H2 metabolismus MeSH
- receptory histaminu metabolismus MeSH
- receptory spřažené s G-proteiny agonisté metabolismus MeSH
- thiomočovina analogy a deriváty farmakologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4-methylhistamine MeSH Prohlížeč
- agonisté histaminu MeSH
- benzimidazoly MeSH
- dimaprit MeSH
- guanidiny MeSH
- histamin MeSH
- histaminový receptor H4 MeSH
- HRH4 protein, human MeSH Prohlížeč
- JNJ 10191584 MeSH Prohlížeč
- methylhistaminy MeSH
- reaktivní formy kyslíku MeSH
- receptory histaminu H2 MeSH
- receptory histaminu MeSH
- receptory spřažené s G-proteiny MeSH
- S-(2-guanidylethyl)isothiourea MeSH Prohlížeč
- thiomočovina MeSH
AIMS: The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. MAIN METHODS: Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol-HRP-H2O2 based CL). KEY FINDINGS: Histamine, 4-methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP-activated whole blood CL in a dose-dependent manner. On the other hand, only VUF8430 was able to inhibit PMA-activated whole blood CL. Ranitidine, but not JNJ10191584, completely reduced the effects of histamine, 4-methylhistamine and dimaprit. The direct scavenging ability of tested compounds was negligible. SIGNIFICANCE: Our results demonstrate that the inhibitory effects of histamine on ROS production in whole blood phagocytes were caused by H2R. Our results also suggest that H4R agonists in concentrations higher than 10(-6)M may also influence ROS production via binding to H2R.
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