Comprehensive portrait of cholesterol containing oxidized membrane
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24565796
DOI
10.1016/j.bbamem.2014.02.006
PII: S0005-2736(14)00070-4
Knihovny.cz E-resources
- Keywords
- Electron paramagnetic resonance, Fluorescence correlation spectroscopy, Lateral diffusion, Molecular dynamics simulations, Oxidized lipids,
- MeSH
- Cell Membrane chemistry MeSH
- Cholesterol chemistry MeSH
- Phospholipids chemistry MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Lipid Bilayers chemistry MeSH
- Membrane Lipids chemistry MeSH
- Oxidation-Reduction MeSH
- Molecular Dynamics Simulation MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cholesterol MeSH
- Phospholipids MeSH
- Lipid Bilayers MeSH
- Membrane Lipids MeSH
Biological membranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation, which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
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