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Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines

. 2014 Aug ; 159 (8) : 2103-7. [epub] 20140306

Language English Country Austria Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.

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KF134123, KF134124, KF134125, KF686810

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