Cell differentiation along multiple pathways accompanied by changes in histone acetylation status
Language English Country Canada Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24697692
DOI
10.1139/bcb-2013-0082
Knihovny.cz E-resources
- MeSH
- Acetylation MeSH
- Cell Differentiation MeSH
- Embryonic Stem Cells cytology metabolism MeSH
- Endoderm cytology metabolism MeSH
- Enterocytes cytology metabolism MeSH
- Epigenesis, Genetic MeSH
- Histone Acetyltransferases metabolism MeSH
- Histones metabolism MeSH
- Humans MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Protein Processing, Post-Translational * MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Histone Acetyltransferases MeSH
- Histones MeSH
Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events, including differentiation. In this study, we analyzed acetylated forms of histones H2A, H2B, and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-, di-, and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes, mono-acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs, we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-, di-, and tri-acetylation of H4 were reduced, manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation, whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.
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