Changes in actin dynamics are involved in salicylic acid signaling pathway
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24767113
DOI
10.1016/j.plantsci.2014.03.002
PII: S0168-9452(14)00046-6
Knihovny.cz E-resources
- Keywords
- Actin dynamics, PR genes, Phosphatidic acid, Salicylic acid,
- MeSH
- Actins metabolism MeSH
- Arabidopsis cytology drug effects genetics metabolism MeSH
- Cyclopentanes metabolism MeSH
- Plant Epidermis cytology drug effects metabolism MeSH
- Salicylic Acid metabolism pharmacology MeSH
- Phosphatidic Acids pharmacology MeSH
- Actin Cytoskeleton drug effects metabolism MeSH
- Oxylipins metabolism MeSH
- Cytoplasmic Streaming drug effects MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Signal Transduction * drug effects genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Cyclopentanes MeSH
- jasmonic acid MeSH Browser
- Salicylic Acid MeSH
- Phosphatidic Acids MeSH
- Oxylipins MeSH
Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the invading pathogen. On the other hand it was also shown that the disruption of actin cytoskeleton leads to the induction of defense response leading to the expression of PATHOGENESIS RELATED proteins (PR). In this study we show that pharmacological actin depolymerization leads to the specific induction of genes in salicylic acid pathway but not that involved in jasmonic acid signaling. Life imaging of leafs of Arabidopsis thaliana with GFP-tagged fimbrin (GFP-fABD2) treated with 1 mM salicylic acid revealed rapid disruption of actin filaments resembling the pattern viewed after treatment with 200 nM latrunculin B. The effect of salicylic acid on actin filament fragmentation was prevented by exogenous addition of phosphatidic acid, which binds to the capping protein and thus promotes actin polymerization. The quantitative evaluation of actin filament dynamics is also presented.
References provided by Crossref.org
Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana
Interconnection between actin cytoskeleton and plant defense signaling
