Chemical composition of Enterococcus faecalis in biofilm cells initiated from different physiologic states
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biofilms growth & development MeSH
- Enterococcus faecalis chemistry physiology MeSH
- Nucleic Acids analysis MeSH
- Proteins analysis MeSH
- Spectrum Analysis, Raman MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nucleic Acids MeSH
- Proteins MeSH
Enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. Its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. However, characteristics of chemical composition of biofilm cells developed by starved E. faecalis cells remain poorly understood. In this study, E. faecalis cells in exponential, stationary, and starvation phases were prepared and separately cultured to form biofilms. Confocal laser scanning microscopy was performed to verify biofilm formation. Raman microscopy was used to investigate the chemical composition of cells within the biofilms. Compared to cells in exponential or stationary phase, starved cells developed biofilms with fewer culturable cells (P < 0.05). Raman analysis revealed that cells produced in the biofilms from starved planktonic cells contained more protein and less nucleic acids than either the corresponding planktonic cells or the cells in biofilms from planktonic cells in exponential or stationary phases, suggesting that biofilm-grown cells from the starvation phase were characterized by increased synthesis of proteins and decreased nucleic acids. This study provides an insight into the chemical composition of biofilm cells developed by starved E. faecalis.
See more in PubMed
J Clin Microbiol. 2001 May;39(5):1763-70 PubMed
J Endod. 2010 Jul;36(7):1241-4 PubMed
J Endod. 2009 Jan;35(1):95-7 PubMed
Environ Microbiol. 2010 Sep;12(9):2496-507 PubMed
Nat Rev Microbiol. 2010 Sep;8(9):623-33 PubMed
Analyst. 2010 Oct;135(10):2512-22 PubMed
Appl Microbiol Biotechnol. 2009 Jul;83(6):1171-82 PubMed
Trends Microbiol. 2001 Jan;9(1):34-9 PubMed
Trends Microbiol. 2009 Sep;17(9):423-30 PubMed
Electrophoresis. 2001 Aug;22(14):2947-54 PubMed
J Endod. 2005 May;31(5):380-6 PubMed
Anal Chem. 2004 Aug 1;76(15):4452-8 PubMed
Anal Bioanal Chem. 2009 Jan;393(1):197-206 PubMed
J Endod. 2010 Apr;36(4):630-5 PubMed
Chemphyschem. 2007 Jan 8;8(1):124-37 PubMed
Adv Appl Microbiol. 2010;70:153-86 PubMed
Biochem Biophys Res Commun. 2010 Jan 1;391(1):664-8 PubMed
J Endod. 1992 Sep;18(9):427-30 PubMed
FEMS Microbiol Lett. 2007 Sep;274(2):232-7 PubMed
J Endod. 2006 Feb;32(2):93-8 PubMed
J Endod. 2008 Jul;34(7):850-4 PubMed
J Endod. 2002 Oct;28(10):689-93 PubMed
Res Microbiol. 2012 Jul;163(6-7):448-56 PubMed
