Comparative proteomic profiling of culture filtrate proteins of less and highly virulent Francisella tularensis strains
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacterial Proteins chemistry MeSH
- Virulence Factors chemistry MeSH
- Francisella tularensis chemistry pathogenicity MeSH
- Culture Media, Conditioned MeSH
- Proteome chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Virulence Factors MeSH
- Culture Media, Conditioned MeSH
- Proteome MeSH
The facultative intracellular bacterium Francisella tularensis is the causal agent of the serious infectious disease tularemia. Despite the dynamic progress, which has been made in last few years, important questions regarding Francisella pathogenicity still remain to be answered. Generally, secreted proteins play an important role in pathogenicity of intracellular microbes. In this study, we investigated the protein composition of the culture filtrate proteins of highly virulent F. tularensis subsp. tularensis, strain SCHU S4 and attenuated F. tularensis subsp. holarctica, live vaccine strain using a comparative proteomic analysis. The majority of proteins identified in this study have been implicated in virulence mechanisms of other pathogens, and several have been categorized as having moonlighting properties; those that have more than one unrelated function. This profiling study of secreted proteins resulted in the unique detection of acid phosphatase (precursor) A (AcpA), β-lactamase, and hypothetical protein FTT0484 in the highly virulent strain SCHU S4 secretome. The release of AcpA may be of importance for F. tularensis subsp. tularensis virulence due to the recently described AcpA role in the F. tularensis escape from phagosomes.
References provided by Crossref.org
Breaking the cellular defense: the role of autophagy evasion in Francisella virulence
Bacterial nucleoid-associated protein HU as an extracellular player in host-pathogen interaction
Control of Francisella tularensis Virulence at Gene Level: Network of Transcription Factors
HU protein is involved in intracellular growth and full virulence of Francisella tularensis
