Aspergillus pragensis sp. nov. discovered during molecular reidentification of clinical isolates belonging to Aspergillus section Candidi
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24951723
DOI
10.1093/mmy/myu022
PII: myu022
Knihovny.cz E-resources
- Keywords
- Aspergillus candidus, Aspergillus tritici, antifungal susceptibility testing, nondermatophyte onychomycosis, otomycosis, polyphasic approach,
- MeSH
- Aspergillus classification genetics isolation & purification MeSH
- Aspergillosis microbiology MeSH
- DNA, Fungal chemistry genetics MeSH
- Phylogeny MeSH
- Calmodulin genetics MeSH
- Humans MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Molecular Sequence Data MeSH
- Mycological Typing Techniques MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Tubulin genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Fungal MeSH
- Calmodulin MeSH
- DNA, Ribosomal Spacer MeSH
- Tubulin MeSH
The identity of nine clinical isolates recovered from Czech patients and presumptively identified as Aspergillus sp. section Candidi based on colony morphology was revised using sequences of β-tubulin, calmodulin gene sequence, and internal transcribed spacer rDNA. Six isolates were from suspected and proven onychomycosis, one from otitis externa, and two associated with probable invasive aspergillosis. The results showed that one Aspergillus candidus isolate was the cause of otitis externa, and both isolates obtained from sputa of patients with probable invasive aspergillosis were reidentified as A. carneus (sect. Terrei) and A. flavus (sect. Flavi). Three isolates from nail scrapings were identified as A. tritici, a verified agent of nondermatophyte onychomycosis. One isolate from toenail was determined to be A. candidus and the two isolates belonged to a hitherto undescribed species, Aspergillus pragensis sp. nov. This species is well supported by phylogenetic analysis based on β-tubulin and calmodulin gene and is distinguishable from other members of sect. Candidi by red-brown reverse on malt extract agar, slow growth on Czapek-Dox agar and inability to grow at 37°C. A secondary metabolite analysis was also provided with comparison of metabolite spectrum to other species. Section Candidi now encompasses five species for which a dichotomous key based on colony characteristics is provided. All clinical isolates were tested for susceptibilities to selected antifungal agents using the Etest and disc diffusion method. Overall sect. Candidi members are highly susceptible to common antifungals.
References provided by Crossref.org
A monograph of Aspergillus section Candidi
Early Diagnosis of Cutaneous Mucormycosis Due to Lichtheimia corymbifera After a Traffic Accident
GENBANK
HG915888, HG915889, HG915890, HG915891, HG916672, HG916673, HG916674, HG916675, HG916676, HG916677, HG916678, HG916679, HG916680
