The identity of nine clinical isolates recovered from Czech patients and presumptively identified as Aspergillus sp. section Candidi based on colony morphology was revised using sequences of β-tubulin, calmodulin gene sequence, and internal transcribed spacer rDNA. Six isolates were from suspected and proven onychomycosis, one from otitis externa, and two associated with probable invasive aspergillosis. The results showed that one Aspergillus candidus isolate was the cause of otitis externa, and both isolates obtained from sputa of patients with probable invasive aspergillosis were reidentified as A. carneus (sect. Terrei) and A. flavus (sect. Flavi). Three isolates from nail scrapings were identified as A. tritici, a verified agent of nondermatophyte onychomycosis. One isolate from toenail was determined to be A. candidus and the two isolates belonged to a hitherto undescribed species, Aspergillus pragensis sp. nov. This species is well supported by phylogenetic analysis based on β-tubulin and calmodulin gene and is distinguishable from other members of sect. Candidi by red-brown reverse on malt extract agar, slow growth on Czapek-Dox agar and inability to grow at 37°C. A secondary metabolite analysis was also provided with comparison of metabolite spectrum to other species. Section Candidi now encompasses five species for which a dichotomous key based on colony characteristics is provided. All clinical isolates were tested for susceptibilities to selected antifungal agents using the Etest and disc diffusion method. Overall sect. Candidi members are highly susceptible to common antifungals.

Bacterial Foodborne Pathogens and Mycology Research Unit National Center for Agricultural Utilization Research Peoria IL USA

Department for Systems Biology Technical University of Denmark Soltofts Plads Building 221 DK 2800 Lyngby Denmark

Department of Botany Faculty of Science Charles University Prague Benátská 2 128 01 Praha 2 Czech Republic Laboratory of Fungal Genetics and Metabolism Institute of Microbiology of the AS CR v v i Vídeňská 1083 142 20 Praha 4 Czech Republic

Department of Dermatology and Venerology 1st Faculty of Medicine Charles University Prague and General University Hospital Prague Czech Republic

Laboratory of Medical Mycology Department of Parasitology Mycology and Mycobacteriology Prague Public Health Institute in Ústí nad Labem Czech Republic

Citace poskytuje Crossref.org

A monograph of Aspergillus section Candidi

Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins

Proven Invasive Pulmonary Aspergillosis in Stem Cell Transplant Recipient Due to Aspergillus sublatus, a Cryptic Species of A. nidulans

Antifungal Susceptibility of the Aspergillus viridinutans Complex: Comparison of Two In Vitro Methods

Phylogeny of xerophilic aspergilli (subgenus Aspergillus) and taxonomic revision of section Restricti

Successful Posaconazole Therapy of Disseminated Alternariosis due to Alternaria infectoria in a Heart Transplant Recipient

From the Tunnels into the Treetops: New Lineages of Black Yeasts from Biofilm in the Stockholm Metro System and Their Relatives among Ant-Associated Fungi in the Chaetothyriales

Early Diagnosis of Cutaneous Mucormycosis Due to Lichtheimia corymbifera After a Traffic Accident

Equine Dermatophytosis due to Trichophyton bullosum, a Poorly Known Zoophilic Dermatophyte Masquerading as T. verrucosum

Zobrazit více v PubMed

GENBANK
HG915888, HG915889, HG915890, HG915891, HG916672, HG916673, HG916674, HG916675, HG916676, HG916677, HG916678, HG916679, HG916680