Mekabu fucoidan: structural complexity and defensive effects against avian influenza A viruses
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25037398
DOI
10.1016/j.carbpol.2014.05.032
PII: S0144-8617(14)00502-5
Knihovny.cz E-resources
- Keywords
- Avian influenza A virus, Immunostimulating effect, Mekabu fucoidan, Spectroscopic methods, Sporophyll Undaria pinnatifida,
- MeSH
- Adjuvants, Immunologic chemistry isolation & purification therapeutic use MeSH
- Antiviral Agents chemistry isolation & purification therapeutic use MeSH
- Ducks virology MeSH
- Seaweed chemistry MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Polysaccharides chemistry isolation & purification therapeutic use MeSH
- Influenza in Birds drug therapy immunology MeSH
- Virus Replication drug effects MeSH
- Antibody Formation drug effects MeSH
- Undaria chemistry MeSH
- Influenza A Virus, H7N2 Subtype drug effects MeSH
- Influenza A virus drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Antiviral Agents MeSH
- fucoidan MeSH Browser
- Polysaccharides MeSH
Fucoidan from the sporophyll (Mekabu) of brown seaweed Undaria pinnatifida (wakame) is interesting due to its various biological activities. Mekabu fucoidan (Mw ∼ 9 kDa) of this study (MF) was previously isolated and characterized by chemical and separation methods including GPC and methylation analysis (Lee, Hayashi, Hashimoto, Nakano, & Hayashi, 2004). It was found that this fucoidan composed of partially sulphated (DS ∼ 0.72) fucose and galactose at approximately equal amounts. Methylation analyses revealed complex structure of MF. However, it has been still unclear about the linkages between units and substitution patterns. To solve these structural tasks, spectroscopic methods (FTIR, FT Raman and NMR) were used in the analysis of native MF and its deesterified derivatives. According to obtained results, this polysaccharide was defined as O-acetylated sulphated fucogalactan. The defensive effects of MF were evaluated on mice infected with avian influenza A viruses (H5N3 and H7N2 subtypes); its efficacy was determined in reducing viral replication and increasing antibody production. Oral administration of MF resulted in suppressing virus yields. In addition, the production of neutralizing antibodies and mucosal IgA in the animals inoculated with the avian influenza A viruses was significantly increased. These results suggested that MF could be used for the prevention of viral infection.
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