Mekabu fucoidan: structural complexity and defensive effects against avian influenza A viruses
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25037398
DOI
10.1016/j.carbpol.2014.05.032
PII: S0144-8617(14)00502-5
Knihovny.cz E-zdroje
- Klíčová slova
- Avian influenza A virus, Immunostimulating effect, Mekabu fucoidan, Spectroscopic methods, Sporophyll Undaria pinnatifida,
- MeSH
- adjuvancia imunologická chemie izolace a purifikace terapeutické užití MeSH
- antivirové látky chemie izolace a purifikace terapeutické užití MeSH
- kachny virologie MeSH
- mořské řasy chemie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- polysacharidy chemie izolace a purifikace terapeutické užití MeSH
- ptačí chřipka u ptáků farmakoterapie imunologie MeSH
- replikace viru účinky léků MeSH
- tvorba protilátek účinky léků MeSH
- Undaria chemie MeSH
- virus chřipky A, podtyp H7N2 účinky léků MeSH
- virus chřipky A účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adjuvancia imunologická MeSH
- antivirové látky MeSH
- fucoidan MeSH Prohlížeč
- polysacharidy MeSH
Fucoidan from the sporophyll (Mekabu) of brown seaweed Undaria pinnatifida (wakame) is interesting due to its various biological activities. Mekabu fucoidan (Mw ∼ 9 kDa) of this study (MF) was previously isolated and characterized by chemical and separation methods including GPC and methylation analysis (Lee, Hayashi, Hashimoto, Nakano, & Hayashi, 2004). It was found that this fucoidan composed of partially sulphated (DS ∼ 0.72) fucose and galactose at approximately equal amounts. Methylation analyses revealed complex structure of MF. However, it has been still unclear about the linkages between units and substitution patterns. To solve these structural tasks, spectroscopic methods (FTIR, FT Raman and NMR) were used in the analysis of native MF and its deesterified derivatives. According to obtained results, this polysaccharide was defined as O-acetylated sulphated fucogalactan. The defensive effects of MF were evaluated on mice infected with avian influenza A viruses (H5N3 and H7N2 subtypes); its efficacy was determined in reducing viral replication and increasing antibody production. Oral administration of MF resulted in suppressing virus yields. In addition, the production of neutralizing antibodies and mucosal IgA in the animals inoculated with the avian influenza A viruses was significantly increased. These results suggested that MF could be used for the prevention of viral infection.
Institute of Organic Chemistry and Biochemistry AS CR Flemingovo sq 2 166 28 Prague 6 Czech Republic
Riken Vitamin Co Ltd 2 9 18 Misaki cho Chiyoda ku Tokyo 101 8370 Japan
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