The increased content of isoflavonoids in dry cell suspension and nutrient medium was observed after application of electric current and AgNO3 on Genista tinctoria L. cultures in vitro. The highest content of genistin (1.7 mg g(- 1) DW - dry weight) was measured in the dry cell suspension culture after 30 min elicitation of 10 V and 6 h cultivation and daidzein content (3.5 mg g(- 1) DW) was measured after 60 min elicitation of 5 V and 24 h cultivation. In the case of AgNO3 elicitation, the content of genistin in dry cell suspension culture (0.5 mg g(- 1) DW) was highest after 48 h of AgNO3 treatment and concentration of 5.9 × 10(- 4) mol/L. The AgNO3 concentration of 5.9 × 10(- 4) mol/L was also the most effective combination for daidzein production (0.9 mg g(- 1) DW) after 168 h. The results of this study show that the secondary metabolites could also be released from G. tinctoria L. cells into the nutrient medium.

Department of Pharmacognosy Faculty of Pharmacy in Hradec Králové Charles University Prague Hradec Králové 500 05 Czech Republic

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