The production of isoflavonoids in Genista tinctoria L. cell suspension culture after abiotic stressors treatment
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Genista tinctoria L, electric current, isoflavonoids, silver nitrate,
- MeSH
- Cell Culture Techniques MeSH
- Silver Nitrate MeSH
- Electricity MeSH
- Stress, Physiological * MeSH
- Genista chemistry MeSH
- Isoflavones analysis biosynthesis chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- daidzein MeSH Browser
- Silver Nitrate MeSH
- genistin MeSH Browser
- Isoflavones MeSH
The increased content of isoflavonoids in dry cell suspension and nutrient medium was observed after application of electric current and AgNO3 on Genista tinctoria L. cultures in vitro. The highest content of genistin (1.7 mg g(- 1) DW - dry weight) was measured in the dry cell suspension culture after 30 min elicitation of 10 V and 6 h cultivation and daidzein content (3.5 mg g(- 1) DW) was measured after 60 min elicitation of 5 V and 24 h cultivation. In the case of AgNO3 elicitation, the content of genistin in dry cell suspension culture (0.5 mg g(- 1) DW) was highest after 48 h of AgNO3 treatment and concentration of 5.9 × 10(- 4) mol/L. The AgNO3 concentration of 5.9 × 10(- 4) mol/L was also the most effective combination for daidzein production (0.9 mg g(- 1) DW) after 168 h. The results of this study show that the secondary metabolites could also be released from G. tinctoria L. cells into the nutrient medium.
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