Antibacterial efficiency of vermiculite/chlorhexidine nanocomposites and results of the in vivo test of harmlessness of vermiculite
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25063143
DOI
10.1016/j.msec.2014.05.054
PII: S0928-4931(14)00337-3
Knihovny.cz E-resources
- Keywords
- Antibacterial activity, Histological examination, In vivo tests, Organovermiculites,
- MeSH
- Anti-Bacterial Agents chemistry pharmacology toxicity MeSH
- Bacteria drug effects MeSH
- Chlorhexidine chemistry pharmacology MeSH
- Gastrointestinal Tract drug effects MeSH
- Rats MeSH
- Nanocomposites chemistry toxicity MeSH
- Rats, Wistar MeSH
- Aluminum Silicates chemistry pharmacology toxicity MeSH
- Mucous Membrane drug effects MeSH
- Materials Testing MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Chlorhexidine MeSH
- Aluminum Silicates MeSH
- vermiculite MeSH Browser
Clay minerals have been proposed as very useful materials for modulating drug delivery. These are the commonly used materials in pharmaceutical production both as inorganic carriers or active agents. We focused on the development of suitable long-acting material for local treatment of oral infection where clay minerals act as inorganic drug carriers. Organovermiculites with antibacterial activity were prepared by ion exchange reactions using different concentrations of chlorhexidine diacetate. The samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and thermal analysis (TGA). The antibacterial activity was evaluated by finding the minimum inhibitory concentration (MIC). All studied organoclays possessed good antibacterial activity after 24h exposure against Escherichia coli, Enterococcus faecalis and particularly against Staphylococcus aureus. Pseudomonas aeruginosa however proved very resistant as only the sample with the highest concentration of CA that successfully inhibited bacterial growth. Furthermore, clay mineral vermiculite was subjected to in vivo toxicological analysis and its influence on gastrointestinal tract during its oral application was investigated. Tissue samples from buccal mucosa, tongue, esophagus, stomach, terminal duodenum, small intestine, caecum, distal colon and liver were subjected to histological examination, both macroscopically and microscopically. Neither systemic nor local reactions were observed. Therefore the toxicity of vermiculite to a mammal model organism can be excluded.
Department of Pathology Masaryk Memorial Cancer Institute Žlutý kopec 7 CZ 65653 Brno Czech Republic
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