Effects of fiber density and plasma modification of nanofibrous membranes on the adhesion and growth of HaCaT keratinocytes
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25085812
DOI
10.1177/0885328214546647
PII: 0885328214546647
Knihovny.cz E-resources
- Keywords
- Tissue engineering, fiber density, fibroblasts, keratinocytes, nanofibers, needle-less electrospinning, plasma-treatment, skin,
- MeSH
- Cell Adhesion physiology MeSH
- Cell Line MeSH
- Keratinocytes cytology physiology MeSH
- Humans MeSH
- Membranes, Artificial * MeSH
- Nanofibers chemistry ultrastructure MeSH
- Bandages MeSH
- Tensile Strength MeSH
- Compressive Strength MeSH
- Plasma Gases chemistry MeSH
- Electroplating MeSH
- Surface Properties MeSH
- Cell Proliferation physiology MeSH
- Materials Testing MeSH
- Hardness MeSH
- Skin, Artificial * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Membranes, Artificial * MeSH
- Plasma Gases MeSH
It may be possible to regulate the cell colonization of biodegradable polymer nanofibrous membranes by plasma treatment and by the density of the fibers. To test this hypothesis, nanofibrous membranes of different fiber densities were treated by oxygen plasma with a range of plasma power and exposure times. Scanning electron microscopy and mechanical tests showed significant modification of nanofibers after plasma treatment. The intensity of the fiber modification increased with plasma power and exposure time. The exposure time seemed to have a stronger effect on modifying the fiber. The mechanical behavior of the membranes was influenced by the plasma treatment, the fiber density, and their dry or wet state. Plasma treatment increased the membrane stiffness; however, the membranes became more brittle. Wet membranes displayed significantly lower stiffness than dry membranes. X-ray photoelectron spectroscopy (XPS) analysis showed a slight increase in oxygen-containing groups on the membrane surface after plasma treatment. Plasma treatment enhanced the adhesion and growth of HaCaT keratinocytes on nanofibrous membranes. The cells adhered and grew preferentially on membranes of lower fiber densities, probably due to the larger area of void spaces between the fibers.
Institute of Physics Academy of Sciences of the Czech Republic Czech Republic
Institute of Physiology Academy of Sciences of the Czech Republic Czech Republic
Institute of Rock Structure and Mechanics Academy of Sciences of the Czech Republic Czech Republic
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