Effects of fiber density and plasma modification of nanofibrous membranes on the adhesion and growth of HaCaT keratinocytes
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25085812
DOI
10.1177/0885328214546647
PII: 0885328214546647
Knihovny.cz E-zdroje
- Klíčová slova
- Tissue engineering, fiber density, fibroblasts, keratinocytes, nanofibers, needle-less electrospinning, plasma-treatment, skin,
- MeSH
- buněčná adheze fyziologie MeSH
- buněčné linie MeSH
- keratinocyty cytologie fyziologie MeSH
- lidé MeSH
- membrány umělé * MeSH
- nanovlákna chemie ultrastruktura MeSH
- obvazy MeSH
- pevnost v tahu MeSH
- pevnost v tlaku MeSH
- plazmové plyny chemie MeSH
- pokovování galvanické MeSH
- povrchové vlastnosti MeSH
- proliferace buněk fyziologie MeSH
- testování materiálů MeSH
- tvrdost MeSH
- umělá kůže * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- membrány umělé * MeSH
- plazmové plyny MeSH
It may be possible to regulate the cell colonization of biodegradable polymer nanofibrous membranes by plasma treatment and by the density of the fibers. To test this hypothesis, nanofibrous membranes of different fiber densities were treated by oxygen plasma with a range of plasma power and exposure times. Scanning electron microscopy and mechanical tests showed significant modification of nanofibers after plasma treatment. The intensity of the fiber modification increased with plasma power and exposure time. The exposure time seemed to have a stronger effect on modifying the fiber. The mechanical behavior of the membranes was influenced by the plasma treatment, the fiber density, and their dry or wet state. Plasma treatment increased the membrane stiffness; however, the membranes became more brittle. Wet membranes displayed significantly lower stiffness than dry membranes. X-ray photoelectron spectroscopy (XPS) analysis showed a slight increase in oxygen-containing groups on the membrane surface after plasma treatment. Plasma treatment enhanced the adhesion and growth of HaCaT keratinocytes on nanofibrous membranes. The cells adhered and grew preferentially on membranes of lower fiber densities, probably due to the larger area of void spaces between the fibers.
Institute of Physics Academy of Sciences of the Czech Republic Czech Republic
Institute of Physiology Academy of Sciences of the Czech Republic Czech Republic
Institute of Rock Structure and Mechanics Academy of Sciences of the Czech Republic Czech Republic
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