Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25217723
DOI
10.1016/j.ijfoodmicro.2014.08.032
PII: S0168-1605(14)00436-X
Knihovny.cz E-zdroje
- Klíčová slova
- Bifidobacterium bifidum, Feces, Isolation, Probiotics, Selective enumeration,
- MeSH
- agar normy MeSH
- bakteriální nálož metody MeSH
- Bifidobacterium genetika izolace a purifikace fyziologie MeSH
- feces mikrobiologie MeSH
- kultivační média normy MeSH
- Lactobacillaceae genetika metabolismus MeSH
- lidé MeSH
- muciny metabolismus MeSH
- mupirocin metabolismus MeSH
- polymerázová řetězová reakce MeSH
- probiotika izolace a purifikace MeSH
- RNA ribozomální 16S genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- agar MeSH
- kultivační média MeSH
- muciny MeSH
- mupirocin MeSH
- RNA ribozomální 16S MeSH
Bifidobacterium bifidum is a bacterial species exclusively found in the human intestinal tract. This species is becoming increasingly popular as a probiotic organism added to lyophilized products. In this study, porcine mucin was used as the sole carbon source for the selective enumeration of B. bifidum in probiotic food additives. Thirty-six bifidobacterial strains were cultivated in broth with mucin. Only 13 strains of B. bifidum utilized the mucin to produce acids. B. bifidum was selectively enumerated in eight probiotic food supplements using agar (MM agar) containing mupirocin (100 mg/L) and mucin (20 g/L) as the sole carbon source. MM agar was fully selective if the B. bifidum species was presented together with Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, and Bifidobacterium longum subsp. longum species and with lactic acid bacteria (lactobacilli, streptococci). Isolated strains of B. bifidum were identified using biochemical, PCR, MALDI-TOF procedures and 16S rRNA gene sequencing. The novel selective medium was also suitable for the isolation of B. bifidum strains from human fecal samples.
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