Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25217723
DOI
10.1016/j.ijfoodmicro.2014.08.032
PII: S0168-1605(14)00436-X
Knihovny.cz E-resources
- Keywords
- Bifidobacterium bifidum, Feces, Isolation, Probiotics, Selective enumeration,
- MeSH
- Agar standards MeSH
- Bacterial Load methods MeSH
- Bifidobacterium genetics isolation & purification physiology MeSH
- Feces microbiology MeSH
- Culture Media standards MeSH
- Lactobacillaceae genetics metabolism MeSH
- Humans MeSH
- Mucins metabolism MeSH
- Mupirocin metabolism MeSH
- Polymerase Chain Reaction MeSH
- Probiotics isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Agar MeSH
- Culture Media MeSH
- Mucins MeSH
- Mupirocin MeSH
- RNA, Ribosomal, 16S MeSH
Bifidobacterium bifidum is a bacterial species exclusively found in the human intestinal tract. This species is becoming increasingly popular as a probiotic organism added to lyophilized products. In this study, porcine mucin was used as the sole carbon source for the selective enumeration of B. bifidum in probiotic food additives. Thirty-six bifidobacterial strains were cultivated in broth with mucin. Only 13 strains of B. bifidum utilized the mucin to produce acids. B. bifidum was selectively enumerated in eight probiotic food supplements using agar (MM agar) containing mupirocin (100 mg/L) and mucin (20 g/L) as the sole carbon source. MM agar was fully selective if the B. bifidum species was presented together with Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, and Bifidobacterium longum subsp. longum species and with lactic acid bacteria (lactobacilli, streptococci). Isolated strains of B. bifidum were identified using biochemical, PCR, MALDI-TOF procedures and 16S rRNA gene sequencing. The novel selective medium was also suitable for the isolation of B. bifidum strains from human fecal samples.
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