Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25336686
DOI
10.1093/jxb/eru422
PII: eru422
Knihovny.cz E-zdroje
- Klíčová slova
- CHASE-containing His kinase, E. coli-based live-cell competitive receptor assay, JBnB library, gene structure, modular protein architecture, phylogenetic analysis,
- MeSH
- Brassica napus genetika metabolismus MeSH
- fylogeneze MeSH
- histidinkinasa MeSH
- molekulární sekvence - údaje MeSH
- proteinkinasy genetika metabolismus MeSH
- proteiny huseníčku MeSH
- regulace genové exprese u rostlin * MeSH
- rostlinné proteiny chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- signální transdukce MeSH
- terciární struktura proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- AHK2 protein, Arabidopsis MeSH Prohlížeč
- AHK3 protein, Arabidopsis MeSH Prohlížeč
- histidinkinasa MeSH
- proteinkinasy MeSH
- proteiny huseníčku MeSH
- rostlinné proteiny MeSH
Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1-BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1-BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1-3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
Citace poskytuje Crossref.org
Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis
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KF621024, KF621025, KF621026, KF621027, KF621028, KF621029, KF621030, KF621031, KF621032, KF621033