Co-expression of the homologous proteases fibroblast activation protein and dipeptidyl peptidase-IV in the adult human Langerhans islets
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buňky vylučující glukagon enzymologie MeSH
- dipeptidylpeptidasa 4 analýza MeSH
- dospělí MeSH
- endopeptidasy MeSH
- glukagon analýza MeSH
- imunohistochemie MeSH
- konfokální mikroskopie MeSH
- Langerhansovy ostrůvky cytologie enzymologie MeSH
- lidé MeSH
- membránové proteiny analýza MeSH
- serinové endopeptidasy analýza MeSH
- želatinasy analýza MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dipeptidylpeptidasa 4 MeSH
- DPP4 protein, human MeSH Prohlížeč
- endopeptidasy MeSH
- fibroblast activation protein alpha MeSH Prohlížeč
- glukagon MeSH
- membránové proteiny MeSH
- serinové endopeptidasy MeSH
- želatinasy MeSH
Fibroblast activation protein (FAP, seprase, EC 3.4.21.B28) and dipeptidyl peptidase-IV (DPP-IV, CD26, EC 3.4.14.5) are homologous serine proteases implicated in the modulation of the bioavailability and thus the function of a number of biologically active peptides. In spite of their generally nonoverlapping expression patterns, DPP-IV and FAP are co-expressed and probably co-regulated in certain cell types suggesting that for some biological processes their functional synergy is essential. By an in situ enzymatic activity assay, we show an abundant DPP-IV-like enzymatic activity sensitive to a highly specific DPP-IV inhibitor sitagliptin and corresponding DPP-IV immunoreactivity in the adult human islets of Langerhans. Moreover, the homologous protease FAP was present in the human endocrine pancreas and was co-expressed with DPP-IV. DPP-IV and FAP were found in the pancreatic alpha cells as determined by the co-localization with glucagon immunoreactivity. In summary, we show abundant enzymatic activity of the canonical DPP-IV (CD26) in Langerhans islets in the natural tissue context and demonstrate for the first time the co-expression of FAP and DPP-IV in pancreatic alpha cells in adult humans. Given their ability to proteolytically modify several biologically active peptides, both proteases have the potential to modulate the paracrine signaling in the human Langerhans islets.
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J Med Chem. 2005 Jan 13;48(1):141-51 PubMed
Biochim Biophys Acta. 2001 Dec 17;1550(2):107-16 PubMed
Clin Pharmacol Ther. 2005 Dec;78(6):675-88 PubMed
Regul Pept. 1999 Nov 30;85(1):9-24 PubMed
J Biol Chem. 2000 Feb 11;275(6):3827-34 PubMed
Diabetologia. 2014 Sep;57(9):1876-83 PubMed
Acta Univ Palacki Olomuc Fac Med. 1991;131:137-43 PubMed
Gastroenterology. 2012 Aug;143(2):459-68 PubMed
J Histochem Cytochem. 1993 Jan;41(1):81-8 PubMed
J Histochem Cytochem. 2005 Sep;53(9):1087-97 PubMed
Regul Pept. 2001 Jan 12;96(3):133-41 PubMed
Int J Biochem Cell Biol. 2004 Mar;36(3):408-21 PubMed
Diabetes. 2011 Sep;60(9):2424-33 PubMed
J Biol Chem. 2002 Aug 9;277(32):29231-41 PubMed
Biochem J. 2006 Jun 1;396(2):391-9 PubMed
Diabetes. 2013 Jul;62(7):2595-604 PubMed
Mol Cell Biochem. 2011 Aug;354(1-2):283-9 PubMed
Diabetes. 2013 Oct;62(10):3316-23 PubMed
Clin Cancer Res. 2007 Jul 15;13(14):4191-200 PubMed
Int J Biochem Cell Biol. 2012 May;44(5):738-47 PubMed
Cancer Res. 2006 May 1;66(9):4652-61 PubMed
Diabetologia. 2012 Dec;55(12):3262-72 PubMed
Oncology. 2008;75(1-2):49-59 PubMed
Endocrinology. 2002 Oct;143(10):3717-26 PubMed
Diabetes Care. 2013 Aug;36 Suppl 2:S145-8 PubMed
Endocrinology. 1999 Nov;140(11):5356-63 PubMed
Endocrinology. 2007 Dec;148(12):5716-23 PubMed
Eur J Intern Med. 2014 Jun;25(5):407-14 PubMed
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2334-9 PubMed
J Histochem Cytochem. 1999 Apr;47(4):489-98 PubMed
J Histochem Cytochem. 1981 Mar;29(3A Suppl):481-93 PubMed
Diabetologia. 2011 Aug;54(8):2067-76 PubMed
FEBS J. 2011 Apr;278(8):1316-32 PubMed
Proteomics Clin Appl. 2014 Jun;8(5-6):454-63 PubMed
Diabetes. 1989 Mar;38(3):338-42 PubMed
Endocrinology. 2011 Aug;152(8):3018-29 PubMed
Cell Metab. 2011 Jul 6;14(1):45-54 PubMed
J Histochem Cytochem. 1996 May;44(5):445-61 PubMed
Diabetes. 2010 May;59(5):1202-10 PubMed
J Histochem Cytochem. 1989 Dec;37(12):1869-75 PubMed
J Exp Med. 1999 Aug 2;190(3):311-22 PubMed
J Biol Chem. 2005 May 20;280(20):19441-4 PubMed
Curr Mol Med. 2012 Dec;12(10):1220-43 PubMed
Pancreas. 2008 Aug;37(2):154-8 PubMed
Biochim Biophys Acta. 1997 Jul 10;1361(1):11-9 PubMed
FASEB J. 1995 Jun;9(9):736-44 PubMed
FEBS Lett. 2008 Mar 5;582(5):819-25 PubMed
Crit Rev Clin Lab Sci. 2003 Jun;40(3):209-94 PubMed
Peptides. 2007 Sep;28(9):1805-13 PubMed
Int J Cancer. 2004 May 10;109(6):855-66 PubMed
Lancet. 2014 Mar 22;383(9922):1068-83 PubMed
J Mol Neurosci. 2012 Oct;48(2):429-40 PubMed
