Experimental determination and computational interpretation of biophysical properties of lipid bilayers enriched by cholesteryl hemisuccinate
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25450348
DOI
10.1016/j.bbamem.2014.10.032
PII: S0005-2736(14)00367-8
Knihovny.cz E-resources
- Keywords
- Cholesterol-mimicking detergents, DPH, Dynamic light scattering, Laurdan, Molecular dynamics simulations, Time-dependent fluorescence shift,
- MeSH
- 2-Naphthylamine analogs & derivatives chemistry MeSH
- Cholesterol chemistry MeSH
- Dihydropyridines chemistry MeSH
- Cholesterol Esters chemistry MeSH
- Phosphatidylcholines chemistry MeSH
- Laurates chemistry MeSH
- Lipid Bilayers chemistry MeSH
- Liposomes chemistry MeSH
- Protons * MeSH
- Molecular Dynamics Simulation MeSH
- Water chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-palmitoyl-2-oleoylphosphatidylcholine MeSH Browser
- 1,2-oleoylphosphatidylcholine MeSH Browser
- 2-Naphthylamine MeSH
- 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylic acid monomethyl ester MeSH Browser
- Cholesterol MeSH
- cholesteryl succinate MeSH Browser
- Dihydropyridines MeSH
- Cholesterol Esters MeSH
- Phosphatidylcholines MeSH
- Laurates MeSH
- laurdan MeSH Browser
- Lipid Bilayers MeSH
- Liposomes MeSH
- Protons * MeSH
- Water MeSH
Cholesteryl hemisuccinate (CHS) is one of the cholesterol-mimicking detergents not observed in nature. It is, however, widely used in protein crystallography, in biochemical studies of proteins, and in pharmacology. Here, we performed an extensive experimental and theoretical study on the behavior of CHS in lipid membranes rich in unsaturated phospholipids. We found that the deprotonated form of CHS (that is the predominant form under physiological conditions) does not mimic cholesterol very well. The protonated form of CHS does better in this regard, but also its ability to mimic the physical effects of cholesterol on lipid membranes is limited. Overall, although ordering and condensing effects characteristic to cholesterol are present in systems containing any form of CHS, their strength is appreciably weaker compared to cholesterol. Based on the considerable amount of experimental and atomistic simulation data, we conclude that these differences originate from the fact that the ester group of CHS does not anchor it in an optimal position at the water-membrane interface. The implications of these findings for considerations of protein-cholesterol interactions are briefly discussed.
References provided by Crossref.org
The Two Faces of the Liquid Ordered Phase
What Does Time-Dependent Fluorescence Shift (TDFS) in Biomembranes (and Proteins) Report on?
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane
