Analysis of 1,2-diol diesters in vernix caseosa by high-performance liquid chromatography - atmospheric pressure chemical ionization mass spectrometry
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25555408
DOI
10.1016/j.chroma.2014.11.075
PII: S0021-9673(14)01873-1
Knihovny.cz E-resources
- Keywords
- Double-bond position, Lipidomics, Mass spectrometry, Neutral lipids, Skin lipids,
- MeSH
- Mass Spectrometry * MeSH
- Humans MeSH
- Fatty Acids analysis chemistry MeSH
- Infant, Newborn MeSH
- Vernix Caseosa chemistry MeSH
- Chromatography, High Pressure Liquid * MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fatty Acids MeSH
Fatty acid diesters of long-chain 1,2-diols (1,2-DDE), or type II wax diesters, were analyzed in the vernix caseosa of a newborn girl. 1,2-DDE were isolated from the total lipid extract by the semipreparative TLC using plates coated with silica gel. Chromatographic separation of the 1,2-DDE molecular species was achieved on the non-aqueous reversed-phase HPLC with two Nova-Pak C18 columns connected in series (a total length of 45cm) and using an acetonitrile-ethyl acetate gradient. 1,2-DDE eluted from the column in the order of their equivalent chain number. The analytes were detected as ammonium adducts by an ion-trap mass spectrometer equipped with an atmospheric pressure chemical ionization source. Their structures were elucidated using tandem mass spectrometry with MS, MS(2) and MS(3) steps in a data-dependent mode. More than two thousand molecular species of 1,2-DDE were identified in 141 chromatographic peaks. The most abundant 1,2-DDE were monounsaturated lipids consisting of a C22 diol and a C18:1 fatty acid together with C16:0, C14:0 or C15:0 fatty acids. The positions of double bonds were characterized by the fragmentation of [M+C3H5N](+) formed in the ion source.
References provided by Crossref.org
Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry
Nonhydroxylated 1-O-acylceramides in vernix caseosa
Cholesteryl esters of ω-(O-acyl)-hydroxy fatty acids in vernix caseosa