Although environmental DNA surveys improve our understanding of biodiversity, interpretation of unidentified lineages is limited by the absence of associated morphological traits and living cultures. Unidentified lineages of marine stramenopiles are called "MAST clades". Twenty-five MAST clades have been recognized: MAST-1 through MAST-25; seven of these have been subsequently discarded because the sequences representing those clades were found to either (1) be chimeric or (2) affiliate within previously described taxonomic groups. Eighteen MAST clades remain without a cellular identity. Moreover, the discarded "MAST-13" has been used in different studies to refer to two different environmental sequence clades. After establishing four cultures representing two different species of heterotrophic stramenopiles and then characterizing their morphology and molecular phylogenetic positions, we determined that the two different species represented the two different MAST-13 clades: (1) a lorica-bearing Bicosoeca kenaiensis and (2) a microaerophilic flagellate previously named "Cafeteria marsupialis". Both species were previously described with only light microscopy; no cultures, ultrastructural data or DNA sequences were available from these species prior to this study. The molecular phylogenetic position of three different "C. marsupialis" isolates was not closely related to the type species of Cafeteria; therefore, we established a new genus for these isolates, Cantina gen. nov.

Center for Computational Sciences and Graduate School of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305 8577 Japan

Department of Zoology Faculty of Science Charles University Prague Prague 128 44 Czech Republic

Japan Agency for Marine Earth Science and Technology Yokosuka Kanagawa 237 0061 Japan

The Departments of Botany and Zoology Beaty Biodiversity Research Centre and Museum University of British Columbia Vancouver British Columbia V6T 1Z4 Canada

Citace poskytuje Crossref.org

Integrated overview of stramenopile ecology, taxonomy, and heterotrophic origin

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