Morphological Identities of Two Different Marine Stramenopile Environmental Sequence Clades: Bicosoeca kenaiensis (Hilliard, 1971) and Cantina marsupialis (Larsen and Patterson, 1990) gen. nov., comb. nov
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25594562
DOI
10.1111/jeu.12207
Knihovny.cz E-resources
- Keywords
- Bicosoecid, MAST, biodiversity, culture-independent molecular methods, environmental DNA sequence surveys, molecular phylogeny, protist, ultrastructure,
- MeSH
- Flagella ultrastructure MeSH
- Phylogeny MeSH
- Stramenopiles classification cytology genetics isolation & purification MeSH
- Heterotrophic Processes MeSH
- Molecular Sequence Data MeSH
- Seawater MeSH
- DNA, Ribosomal genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 18S MeSH
Although environmental DNA surveys improve our understanding of biodiversity, interpretation of unidentified lineages is limited by the absence of associated morphological traits and living cultures. Unidentified lineages of marine stramenopiles are called "MAST clades". Twenty-five MAST clades have been recognized: MAST-1 through MAST-25; seven of these have been subsequently discarded because the sequences representing those clades were found to either (1) be chimeric or (2) affiliate within previously described taxonomic groups. Eighteen MAST clades remain without a cellular identity. Moreover, the discarded "MAST-13" has been used in different studies to refer to two different environmental sequence clades. After establishing four cultures representing two different species of heterotrophic stramenopiles and then characterizing their morphology and molecular phylogenetic positions, we determined that the two different species represented the two different MAST-13 clades: (1) a lorica-bearing Bicosoeca kenaiensis and (2) a microaerophilic flagellate previously named "Cafeteria marsupialis". Both species were previously described with only light microscopy; no cultures, ultrastructural data or DNA sequences were available from these species prior to this study. The molecular phylogenetic position of three different "C. marsupialis" isolates was not closely related to the type species of Cafeteria; therefore, we established a new genus for these isolates, Cantina gen. nov.
Department of Zoology Faculty of Science Charles University Prague Prague 128 44 Czech Republic
Japan Agency for Marine Earth Science and Technology Yokosuka Kanagawa 237 0061 Japan
References provided by Crossref.org
Integrated overview of stramenopile ecology, taxonomy, and heterotrophic origin
GENBANK
KM816645, KM816649
