Diplacone and mimulone ameliorate dextran sulfate sodium-induced colitis in rats
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25623260
DOI
10.1016/j.fitote.2015.01.012
PII: S0367-326X(15)00014-3
Knihovny.cz E-resources
- Keywords
- Dextran sulfate sodium (DSS), Diplacone, Diplacone (PubChem CID: 14539948), Geranylated flavanones, In vivo, Mimulone, Mimulone (PubChem CID: 5716903), Sulfasalazine (PubChem CID: 5353980), Ulcerative colitis,
- MeSH
- Cyclooxygenase 2 metabolism MeSH
- Flavanones pharmacology MeSH
- Catalase metabolism MeSH
- Colitis chemically induced drug therapy MeSH
- Colon drug effects physiopathology MeSH
- Matrix Metalloproteinase 2 metabolism MeSH
- Disease Models, Animal MeSH
- Molecular Structure MeSH
- Rats, Wistar MeSH
- Dextran Sulfate adverse effects MeSH
- Superoxide Dismutase metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cyclooxygenase 2 MeSH
- diplacone MeSH Browser
- Flavanones MeSH
- Catalase MeSH
- Matrix Metalloproteinase 2 MeSH
- mimulone MeSH Browser
- Mmp2 protein, rat MeSH Browser
- Ptgs2 protein, rat MeSH Browser
- Dextran Sulfate MeSH
- Superoxide Dismutase MeSH
- superoxide dismutase 2 MeSH Browser
Diplacone (1) and mimulone (2), two geranylated flavanones, have previously shown anti-inflammatory and antiradical activity in vitro. The present study aimed to evaluate their activity in vivo on a model of colitis induced in Wistar rats by an oral administration of dextran sulfate sodium (DSS). Diplacone (1) and mimulone (2) were administered at a bolus dose of 25mg/kg by gastric gavage 48 and 24h prior to the induction of colitis by DSS and every 24h on the following days of the experiment. The effect of the treatment was assessed by monitoring the disease activity index (DAI), histopathological examination, evaluation of the weight and length of the colon and by analysis of the levels and activities of cyclooxygenase-2 (COX-2), matrix metalloproteinase-2 (MMP2), superoxide dismutase-2 (SOD2), and catalase (CAT) in the inflamed tissue. Administration of the test compounds prior and after induction of colitis ameliorated the symptoms of colitis (diarrhea, presence of the blood in the stool) and delayed their onset. The ability of compounds 1 and 2 to reduce the levels of COX-2 and to increase the ratio of pro-MMP2/MMP2 activity correlates with the values of the DAI. The lowering of the levels of the antioxidant enzymes SOD2 and CAT reflects the ability of the test compounds to scavenge reactive oxygen species.
References provided by Crossref.org
Prenylated Flavonoids in Topical Infections and Wound Healing
Prenylated flavonoid morusin protects against TNBS-induced colitis in rats
