Surface passivation improves the synthesis of highly stable and specific DNA-functionalized gold nanoparticles with variable DNA density
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25756758
DOI
10.1021/acsami.5b01191
Knihovny.cz E-zdroje
- Klíčová slova
- DNA functionalization, gold nanoparticles, oligo ethylene glycol, salt-stability, specificity, surface passivation, variable DNA density,
- MeSH
- ethylenglykol chemie MeSH
- fluorescence MeSH
- hybridizace nukleových kyselin MeSH
- jednovláknová DNA chemie genetika MeSH
- kovové nanočástice chemie MeSH
- oligonukleotidy chemie genetika MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ethylenglykol MeSH
- jednovláknová DNA MeSH
- oligonukleotidy MeSH
- zlato MeSH
We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA-DNA hybridization, as shown from UV-vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.
†Elettra Sincrotrone Trieste s s 14 km 163 5 in Area Science Park 34149 Basovizza Trieste Italy
⊥INSTM ST Unit s s 14 km 163 5 in Area Science Park 34149 Basovizza Trieste Italy
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