Molecular characterization of 'Candidatus Rickettsia vini' in Ixodes arboricola from the Czech Republic and Slovakia
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25769386
DOI
10.1016/j.ttbdis.2015.02.006
PII: S1877-959X(15)00032-1
Knihovny.cz E-resources
- Keywords
- Birds, Central Europe, Ixodes arboricola, Rickettsia, Ticks, ‘Candidatus Rickettsia vini’,
- MeSH
- Bacterial Proteins genetics MeSH
- Phylogeny MeSH
- Tick Infestations epidemiology veterinary MeSH
- Ixodes microbiology MeSH
- Molecular Sequence Data MeSH
- Multilocus Sequence Typing veterinary MeSH
- Nymph MeSH
- Passeriformes MeSH
- Rickettsia classification genetics isolation & purification MeSH
- Rickettsia Infections epidemiology microbiology veterinary MeSH
- Base Sequence MeSH
- Trees MeSH
- Bacterial Typing Techniques veterinary MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Slovakia epidemiology MeSH
- Names of Substances
- Bacterial Proteins MeSH
The aim of this study was to analyze the prevalence of rickettsiae in the tree-hole tick Ixodes arboricola in the Czech Republic and Slovakia. During May to September of 2009 and 2013, bird boxes belonging to three different areas were screened for ticks. In total, 454 nestlings and 109 nests of 10 hole-breeding bird species were examined. Ticks were found on Ficedula albicollis, Parus major, Cyanistes caeruleus and Sitta europaea and/or in their nests. In total, 166 ticks (17 nymphs, 10 males and 139 females) were found at 3 areas (arithmetic mean±standard error: 55.3±45.9). All ticks were tested for the presence of Rickettsia species by polymerase chain reaction targeting the rickettsial genes gltA, ompA, ompB and htrA and amplicon sequencing. All individuals except 3 nymphs were infected with 'Candidatus Rickettsia vini'. Multilocus sequence typing showed closest proximity to Rickettsia japonica and Rickettsia heilongjiangensis cluster. The presence of 'Ca. R. vini' is reported for the first time in Slovakia.
Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic
Institute of Forest Ecology Slovak Academy of Sciences Zvolen Slovakia
References provided by Crossref.org
Rickettsia vini n. sp. (Rickettsiaceae) infecting the tick Ixodes arboricola (Acari: Ixodidae)
Ticks and tick-borne pathogens in wild birds in Greece
