Post-Translational Modifications of Histones in Human Sperm
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25808548
DOI
10.1002/jcb.25170
Knihovny.cz E-resources
- Keywords
- EPIGENETICS, HISTONES, HUMAN SPERM, MICROSCOPY, PROTAMINE P2,
- MeSH
- Acetylation MeSH
- Chromatin genetics MeSH
- Histone-Lysine N-Methyltransferase biosynthesis genetics MeSH
- Histone Methyltransferases MeSH
- Histones genetics metabolism MeSH
- Humans MeSH
- Methylation MeSH
- Protein Processing, Post-Translational genetics MeSH
- Amino Acid Sequence MeSH
- Spermatozoa growth & development metabolism MeSH
- Tandem Mass Spectrometry MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- Histone-Lysine N-Methyltransferase MeSH
- Histone Methyltransferases MeSH
- Histones MeSH
We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the level of acetylated (ac) histones H4 was relatively stable in the sperm head fractions, as demonstrated by western blot analysis. Sperm heads with lower levels of P2 exhibited lower levels of H3K9ac, H3K9me1, H3K27me3, H3K36me3, and H3K79me1. A very strong correlation was observed between the levels of P2 and H3K9me2. FLIM-FRET analysis additionally revealed that acetylated histones H4 are not only parts of sperm chromatin but also appear in a non-integrated form. Intriguingly, H4ac and H3K27me3 were detected in sperm tail fractions via western blot analysis. An appearance of specific histone H3 and H4 acetylation and H3 methylation in sperm tail fractions was also confirmed by both LC-MS/MS and MALDI-TOF MS analysis. Taken together, these data indicate that particular post-translational modifications of histones are uniquely distributed in human sperm, and this distribution varies among individuals and among the sperm of a single individual.
References provided by Crossref.org
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