Ionizing radiation increases primary cilia incidence and induces multiciliation in C2C12 myoblasts
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25808704
DOI
10.1002/cbin.10462
Knihovny.cz E-zdroje
- Klíčová slova
- cell line, ionizing radiation, multiple cilia, myoblast, primary cilium, serum starvation stress,
- MeSH
- apoptóza účinky záření MeSH
- buněčná diferenciace účinky záření MeSH
- buněčné linie MeSH
- centrozom účinky záření MeSH
- cilie účinky záření MeSH
- dvouřetězcové zlomy DNA MeSH
- ionizující záření MeSH
- kaspasy metabolismus MeSH
- lidé MeSH
- myoblasty cytologie účinky léků metabolismus MeSH
- poškození DNA MeSH
- proliferace buněk účinky záření MeSH
- vztah dávky záření a odpovědi MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kaspasy MeSH
Primary cilia act as physical-chemical sensors and their functions include the perception of the extracellular milieu, regulation of organogenesis, and cell polarity. In general, these cells are monociliated and the single cilium possesses diverse receptors and channels which are involved in morphogenesis and growth signaling, and are, therefore, important for cell proliferation and differentiation. In this study, we used an in vitro model of C2C12 myoblasts to evaluate the effect of DNA damage induced by gamma ionizing radiation on primary cilia incidence. A significantly higher number of ciliated cells were observed after 1 day post-irradiation with 2-20 Gy when compared with non-irradiated cells. After 3 days post-irradiation, the cilia incidence in cells had decreased slightly when treated with 2, 6, and 10 Gy, although an increase in incidence rate was observed in cells treated with 20 Gy. Multi-ciliated cells were also detected in myoblasts irradiated with 10 and 20 Gy but not in non-irradiated cells or after low irradiation (2-6 Gy). Irradiation also caused a dose-dependent decrease in cell viability and proliferation and corresponding cell cycle arrest. Furthermore, an activation of caspases 3/7, 8, and 9 was observed after higher radiation (10 and 20 Gy) with increased apoptosis. Together, our results show that irradiation by gamma rays promotes myoblast ciliogenesis, with pronounced effects observed after 3 days post-irradiation. We conclude that irradiation doses of 10 and 20 Gy are sufficient to induce cell death and are responsible for the formation of multiple cilia originating from multiple basal bodies.
Citace poskytuje Crossref.org
The toxic effect of cytostatics on primary cilia frequency and multiciliation
