Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) ( = CCM 8529(T) = LMG 28327(T)).

Czech Collection of Microorganisms Department of Experimental Biology Faculty of Science Masaryk University Kamenice 5 Bld A25 625 00 Brno Czech Republic

Department of Biochemistry and Microbiology Faculty of Sciences Ghent University K L Ledeganckstraat 35 9000 Ghent Belgium

Department of Microbiology and Infection Control Universitair Ziekenhuis Brussel Vrije Universiteit Brussel Laarbeeklaan 101 1090 Brussels Belgium

Division of Genetics and Molecular Biology Department of Experimental Biology Faculty of Science Masaryk University Kotlárˇská 2 611 37 Brno Czech Republic

Reference Laboratory for Staphylococci National Institute of Public Health Šrobárova 48 100 42 Prague 10 Czech Republic

Citace poskytuje Crossref.org

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

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