Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25829332
DOI
10.1099/ijs.0.000220
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial MeSH
- Chaperonin 60 genetics MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny * MeSH
- Nucleic Acid Hybridization MeSH
- Humans MeSH
- Fatty Acids chemistry MeSH
- Molecular Sequence Data MeSH
- Oxidoreductases genetics MeSH
- Ribotyping MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Staphylococcus classification genetics isolation & purification MeSH
- Base Composition MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Chaperonin 60 MeSH
- DNA, Bacterial MeSH
- Fatty Acids MeSH
- Oxidoreductases MeSH
- RNA, Ribosomal, 16S MeSH
Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) ( = CCM 8529(T) = LMG 28327(T)).
References provided by Crossref.org
GENBANK
KM873669, KM873670, KM873671, KM873672, KM873673, KM873674, KM873675, KM873676, KM873677, KM873678, KM873679, KM873680, KM873681, KM873682, KM873683, KM873684, KM873685, KM873686, KM873687, KM873688, KM873689, KM873690, KM873691, KM873692, KM873693, KM873694, KM873695, KM873696, KM873697, KM873698, KM873699, KM873700, KM873701, KM873702, KM873703, KM873704, KM873705, KM873706, KM873707, KM873708, KM873709, KM873710
