IMP-8 metallo-β-lactamase was identified in Klebsiella pneumoniae sequence type 252 (ST252), isolated in a Portuguese hospital in 2009. blaIMP-8 was the first gene cassette of a novel class 3 integron, In1144, also carrying the blaGES-5, blaBEL-1, and aacA4 cassettes. In1144 was located on a ColE1-like plasmid, pKP-M1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other RNA-priming plasmids, such as pJHCMW1. In1144 and pKP-M1144 represent an interesting case of evolution of resistance determinants in Gram-negative bacteria.

Centro Hospitalar Trás os Montes e Alto Douro Vila Real Portugal

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Faculty of Medicine and University Hospital in Plzen Charles University Prague Plzen Czech Republic

Faculty of Medicine and University Hospital in Plzen Charles University Prague Plzen Czech Republic Biomedical Center Faculty of Medicine in Plzen Charles University Prague Plzen Czech Republic

Faculty of Medicine and University Hospital in Plzen Charles University Prague Plzen Czech Republic National Medicines Institute Warsaw Poland

National Medicines Institute Warsaw Poland

University Medical Center Utrecht Utrecht The Netherlands

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