The sequence type 11 Klebsiella pneumoniae strain Kpn-3002cz was confirmed to harbor two NDM-1-encoding plasmids, pB-3002cz and pS-3002cz. pB-3002cz (97,649 bp) displayed extensive sequence similarity with the blaNDM-1-carrying plasmid pKPX-1. pS-3002cz (73,581 bp) was found to consist of an IncR-related sequence (13,535 bp) and a mosaic region (60,046 bp). A 40,233-bp sequence of pS-3002cz was identical to the mosaic region of pB-3002cz, indicating the en bloc acquisition of the NDM-1-encoding region from one plasmid by the other.

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Department of Hospital Epidemiology Central Military Hospital Prague Czech Republic

Department of Microbiology Faculty of Medicine and University Hospital in Plzen Charles University Prague Plzen Czech Republic

Department of Microbiology Faculty of Medicine and University Hospital in Plzen Charles University Prague Plzen Czech Republic Biomedical Center Faculty of Medicine in Plzen Charles University Prague Plzen Czech Republic

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