Iron(II) supramolecular helicates condense plasmid DNA and inhibit vital DNA-related enzymatic activities
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- DNA, condensation, enzymes, helical structures, inhibition,
- MeSH
- DNA-Directed RNA Polymerases antagonists & inhibitors MeSH
- DNA Topoisomerases, Type I metabolism MeSH
- Escherichia coli enzymology MeSH
- Enzyme Inhibitors chemistry pharmacology MeSH
- Topoisomerase I Inhibitors chemistry pharmacology MeSH
- DNA, Circular chemistry metabolism ultrastructure MeSH
- Models, Molecular MeSH
- Plasmids chemistry metabolism ultrastructure MeSH
- DNA Restriction Enzymes antagonists & inhibitors MeSH
- Ferrous Compounds chemistry pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Directed RNA Polymerases MeSH
- DNA Topoisomerases, Type I MeSH
- Enzyme Inhibitors MeSH
- Topoisomerase I Inhibitors MeSH
- DNA, Circular MeSH
- DNA Restriction Enzymes MeSH
- Ferrous Compounds MeSH
The dinuclear iron(II) supramolecular helicates [Fe2 L3 ]Cl4 (L=C25 H20 N4 ) bind to DNA through noncovalent (i.e., hydrogen-bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe2 L3 ]Cl4 do not form intermolecular aggregates typically formed by other condensing agents, such as spermidine or spermine. The helicates inhibit the activity of several DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2 L3 ]Cl4 helicates on DNA-related enzymatic activities have been proposed.
References provided by Crossref.org
Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
