Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Bim, Puma, TUNEL, apoptosis, development, hair follicle, immunofluorescence,
- MeSH
- apoptóza fyziologie MeSH
- barvení a značení metody MeSH
- fluorescenční protilátková technika metody MeSH
- koncové značení zlomů DNA in situ metody MeSH
- membránové proteiny metabolismus MeSH
- myši MeSH
- nádorové supresorové proteiny metabolismus MeSH
- protein BCL2L11 MeSH
- proteiny regulující apoptózu metabolismus MeSH
- protoonkogenní proteiny metabolismus MeSH
- vlasový folikul cytologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Bcl2l11 protein, mouse MeSH Prohlížeč
- membránové proteiny MeSH
- nádorové supresorové proteiny MeSH
- protein BCL2L11 MeSH
- proteiny regulující apoptózu MeSH
- protoonkogenní proteiny MeSH
- PUMA protein, mouse MeSH Prohlížeč
Apoptosis in hair follicles often is studied under pathological conditions; little is known about apoptotic mechanisms during normal hair follicle formation and maintenance. We investigated proteins of intrinsic apoptotic pathway, Bim and Puma, during hair follicle development and the first catagen stage using immunofluorescence to describe their expression patterns and to correlate them with apoptosis as determined by TUNEL assay. Both proteins were found in developing follicles. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. Our findings suggest that these primary apoptotic molecules participate in postnatal development and maintenance of hair follicles.
b Department of Experimental Biology Masaryk University Brno Czech Republic
c Department of Physiology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic
Institute of Animal Physiology and Genetics AS CR Brno Czech Republic
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