Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Bim, Puma, TUNEL, apoptosis, development, hair follicle, immunofluorescence,
- MeSH
- Apoptosis physiology MeSH
- Staining and Labeling methods MeSH
- Fluorescent Antibody Technique methods MeSH
- In Situ Nick-End Labeling methods MeSH
- Membrane Proteins metabolism MeSH
- Mice MeSH
- Tumor Suppressor Proteins metabolism MeSH
- Bcl-2-Like Protein 11 MeSH
- Apoptosis Regulatory Proteins metabolism MeSH
- Proto-Oncogene Proteins metabolism MeSH
- Hair Follicle cytology metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bcl2l11 protein, mouse MeSH Browser
- Membrane Proteins MeSH
- Tumor Suppressor Proteins MeSH
- Bcl-2-Like Protein 11 MeSH
- Apoptosis Regulatory Proteins MeSH
- Proto-Oncogene Proteins MeSH
- PUMA protein, mouse MeSH Browser
Apoptosis in hair follicles often is studied under pathological conditions; little is known about apoptotic mechanisms during normal hair follicle formation and maintenance. We investigated proteins of intrinsic apoptotic pathway, Bim and Puma, during hair follicle development and the first catagen stage using immunofluorescence to describe their expression patterns and to correlate them with apoptosis as determined by TUNEL assay. Both proteins were found in developing follicles. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. Our findings suggest that these primary apoptotic molecules participate in postnatal development and maintenance of hair follicles.
b Department of Experimental Biology Masaryk University Brno Czech Republic
c Department of Physiology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic
Institute of Animal Physiology and Genetics AS CR Brno Czech Republic
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