A high frequency of viral agents yet absence of Borrelia burgdorferi is seen within the myocardium of subjects with normal left ventricular systolic function: an electron microscopy study
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26205424
DOI
10.1007/s12223-015-0417-8
PII: 10.1007/s12223-015-0417-8
Knihovny.cz E-resources
- MeSH
- Antiviral Agents adverse effects therapeutic use MeSH
- Borrelia burgdorferi genetics isolation & purification physiology ultrastructure MeSH
- Cardiomyopathy, Dilated microbiology physiopathology virology MeSH
- Microscopy, Electron MeSH
- Ventricular Function, Left MeSH
- Blood Pressure MeSH
- Middle Aged MeSH
- Humans MeSH
- Lyme Disease classification microbiology physiopathology MeSH
- Myocardium ultrastructure MeSH
- Prospective Studies MeSH
- Aged MeSH
- Heart microbiology physiopathology virology MeSH
- Virus Diseases complications drug therapy virology MeSH
- Viruses classification genetics isolation & purification ultrastructure MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antiviral Agents MeSH
A wide range of viral agents is associated with the development of acute myocarditis and its possible chronic sequela, dilated cardiomyopathy (DCM). There is also increasing evidence that Borrelia burgdorferi (Bb) is associated with DCM in endemic regions for Bb infection. This study sought to use electron microscopy to prospectively analyze the presence of viruses and Bb within the myocardium of 40 subjects with preserved left ventricular (LV) ejection fraction and 40 patients with new-onset unexplained DCM during the same time period. Virus particles were found within the myocardium of 23 subjects (58%) of both cohorts studied, yet there was no statistically significant difference in virus family presence between those with DCM versus those with preserved LV systolic function. In contrast, Bb was detected only in those subjects with DCM (0 versus 5 subjects; p ˂ 0.05). Polymerase chain reaction was performed on samples from patients who were positive for Bb according to electron microscopy, and Bb was confirmed in 4 out of 5 individuals. Our results demonstrate that the prevalence of viral particles does not differ between subjects with preserved LV systolic function versus those with DCM and therefore suggests that the mere presence of a viral agent within the myocardium is not sufficient to establish a clear link with the development of DCM. In contrast, the presence of Bb was found only within myocardial samples of patients with DCM; this finding supports the idea of a causal relationship between Bb infection and DCM development.
International Clinical Research Center St Anne's University Hospital in Brno Brno Czech Republic
The Heart Hospital University College London London Great Britain
The National Institute of Public Health Prague Czech Republic
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