Instrument platforms for nano liquid chromatography
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
26265002
DOI
10.1016/j.chroma.2015.07.090
PII: S0021-9673(15)01078-X
Knihovny.cz E-zdroje
- Klíčová slova
- Chromatographic separation, Nano LC platforms, Nano liquid chromatography, Nanoflow delivery, Splitless gradient generation,
- MeSH
- chromatografie kapalinová přístrojové vybavení metody MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- mikrochemie MeSH
- nanotechnologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
The history of liquid chromatography started more than a century ago and miniaturization and automation are two leading trends in this field. Nanocolumn liquid chromatography (nano LC) and largely synonymous capillary liquid chromatography (capillary LC) are the most recent results of this process where miniaturization of column dimensions and sorbent particle size play crucial role. Very interesting results achieved in the research of extremely miniaturized LC columns at the end of the last century lacked distinctive raison d'être and only advances in mass spectrometry brought a real breakthrough. Configuration of nano LC-electrospray ionization mass spectrometry (LC-ESI-MS) has become a basic tool in bioanalytical chemistry, especially in proteomics. This review discusses and summarizes past and current trends in the realization of nano liquid chromatography (nano LC) platforms. Special attention is given to the mobile phase delivery under nanoflow rates (isocratic, gradient) and sample injection to the nanocolumn. Available detection techniques applied in nano LC separations are also briefly discussed. We followed up the key themes from the original scientific reports over gradual improvements up to the contemporary commercial solutions.
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