Enhanced sensitivity of pHluorin-based monitoring of intracellular pH changes achieved through synchronously scanned fluorescence spectra
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- fluorescenční spektrometrie MeSH
- koncentrace vodíkových iontů MeSH
- Saccharomyces cerevisiae chemie cytologie metabolismus MeSH
- zelené fluorescenční proteiny chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- PHluorin MeSH Prohlížeč
- zelené fluorescenční proteiny MeSH
Since its introduction in 1998, genetically encoded pH-sensitive sensor ratiometric pHluorin proved to be a valuable tool for cell physiology studies. Here, we show how the sensitivity of pHluorin-based monitoring of intracellular pH changes performed with cell suspensions can be enhanced by using synchronously scanned fluorescence spectroscopy. In the suspensions of S. cerevisiae cells subjected to varying extracellular pH values, we have been able to measure statistically significant changes in intracellular pH of less than 0.1 unit, which were not detectable using a standard ratiometric approach.
Citace poskytuje Crossref.org
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