H+ translocation by weak acid uncouplers is independent of H+ electrochemical gradient
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
28900787
DOI
10.1007/s10863-017-9724-x
PII: 10.1007/s10863-017-9724-x
Knihovny.cz E-resources
- Keywords
- CCCP, Electrochemical H+ gradient, Mode of action, Saccharomyces cerevisiae, Weak acid uncouplers, pHluorin,
- MeSH
- Biological Transport drug effects MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone pharmacology MeSH
- Hydrogen-Ion Concentration drug effects MeSH
- Membrane Potentials * drug effects MeSH
- Protons * MeSH
- Uncoupling Agents pharmacology MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Carbonyl Cyanide m-Chlorophenyl Hydrazone MeSH
- Protons * MeSH
- Uncoupling Agents MeSH
According to the common view, weak acid uncouplers increase proton conductance of biological (and phospholipid bilayer) membranes, thus effecting H+ fluxes driven by their electrochemical gradients. Under certain conditions, however, uncouplers can induce unexpected effects opposite to the dissipation of H+ gradients. Results are presented here demonstrating CCCP-induced proton influx into Saccharomyces cerevisiae cytosol driven by the electrochemical potentials of CCCP and its CCCP- anions, independent of electrochemical H+-gradient. Another view of week acid uncouplers' action is proposed that is logically consistent with these observations.
Faculty of Mathematics and Physics Charles University Ke Karlovu 3 12116 Prague Czech Republic
Institute of Cellular and Molecular Botany University of Bonn Kirschallee 1 53115 Bonn Germany
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