Ellagic Acid-Changed Epigenome of Ribosomal Genes and Condensed RPA194-Positive Regions of Nucleoli in Tumour Cells
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26333121
DOI
10.14712/fb2015061020049
PII: file/5772/fb2015a0007.pdf
Knihovny.cz E-resources
- MeSH
- Tumor Suppressor p53-Binding Protein 1 MeSH
- Cell Division drug effects MeSH
- Cell Nucleolus chemistry drug effects ultrastructure MeSH
- Chromosomal Proteins, Non-Histone analysis MeSH
- Dactinomycin pharmacology MeSH
- Epigenesis, Genetic drug effects MeSH
- G2 Phase drug effects MeSH
- Guanylate Cyclase analysis antagonists & inhibitors MeSH
- HeLa Cells chemistry drug effects MeSH
- Histones analysis metabolism MeSH
- Intracellular Signaling Peptides and Proteins analysis MeSH
- Ellagic Acid pharmacology MeSH
- Humans MeSH
- Methylation MeSH
- Neoplasm Proteins analysis MeSH
- Nucleolus Organizer Region chemistry drug effects ultrastructure MeSH
- DNA Damage MeSH
- Protein Processing, Post-Translational drug effects MeSH
- Promoter Regions, Genetic MeSH
- DNA, Ribosomal drug effects genetics MeSH
- RNA Polymerase I analysis MeSH
- CARD Signaling Adaptor Proteins analysis antagonists & inhibitors MeSH
- Pol1 Transcription Initiation Complex Proteins analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Tumor Suppressor p53-Binding Protein 1 MeSH
- CARD11 protein, human MeSH Browser
- Chromosomal Proteins, Non-Histone MeSH
- Dactinomycin MeSH
- fibrillarin MeSH Browser
- Guanylate Cyclase MeSH
- H2AX protein, human MeSH Browser
- Histones MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Ellagic Acid MeSH
- Neoplasm Proteins MeSH
- DNA, Ribosomal MeSH
- RNA Polymerase I MeSH
- RPA194 protein, human MeSH Browser
- CARD Signaling Adaptor Proteins MeSH
- TP53BP1 protein, human MeSH Browser
- transcription factor UBF MeSH Browser
- Pol1 Transcription Initiation Complex Proteins MeSH
We studied the effect of ellagic acid (EA) on the morphology of nucleoli and on the pattern of major proteins of the nucleolus. After EA treatment of HeLa cells, we observed condensation of nucleoli as documented by the pattern of argyrophilic nucleolar organizer regions (AgNORs). EA also induced condensation of RPA194-positive nucleolar regions, but no morphological changes were observed in nucleolar compartments positive for UBF1/2 proteins or fibrillarin. Studied morphological changes induced by EA were compared with the morphology of control, non-treated cells and with pronounced condensation of all nucleolar domains caused by actinomycin D (ACT-D) treatment. Similarly as ACT-D, but in a lesser extent, EA induced an increased number of 53BP1-positive DNA lesions. However, the main marker of DNA lesions, γH2AX, was not accumulated in body-like nuclear structures. An increased level of γH2AX was found by immunofluorescence and Western blots only after EA treatment. Intriguingly, the levels of fibrillarin, UBF1/2 and γH2AX were increased at the promoters of ribosomal genes, while 53BP1 and CARM1 levels were decreased by EA treatment at these genomic regions. In the entire genome, EA reduced H3R17 dimethylation. Taken together, ellagic acid is capable of significantly changing the nucleolar morphology and protein levels inside the nucleolus.
Epigenetic MedChem Lab Università di Salerno Dipartimento di Farmacia Fisciano Salerno Italy
Institute of Biophysics Academy of Sciences of the Czech Republic v v i Brno Czech Republic
References provided by Crossref.org
Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction
