Morphological and Molecular Diversity of the Neglected Genus Rhizomastix Alexeieff, 1911 (Amoebozoa: Archamoebae) with Description of Five New Species
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26333803
DOI
10.1111/jeu.12266
Knihovny.cz E-resources
- Keywords
- Archamoebae, morphology, phylogeny, ultrastructure,
- MeSH
- Actins genetics MeSH
- Amoebozoa classification genetics isolation & purification ultrastructure MeSH
- Species Specificity MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- Geologic Sediments parasitology MeSH
- Microscopy MeSH
- DNA, Protozoan analysis MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Fresh Water parasitology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- DNA, Protozoan MeSH
The genus Rhizomastix is a poorly known group of amoeboid heterotrophic flagellates living as intestinal commensals of insects, amphibians or reptiles, and as inhabitants of organic freshwater sediments. Eleven Rhizomastix species have been described so far, but DNA sequences from only a single species have been published. Recently, phylogenetic analyses confirmed a previous hypothesis that the genus belongs to the Archamoebae; however, its exact position therein remains unclear. In this study we cultured nine strains of Rhizomastix, both endobiotic and free-living. According to their light-microscopic morphology and SSU rRNA and actin gene analyses, the strains represent five species, of which four are newly described here: R. bicoronata sp. nov., R. elongata sp. nov., R. vacuolata sp. nov. and R. varia sp. nov. In addition, R. tipulae sp. nov., living in the intestine of crane flies, is separated from the type species, R. gracilis. We also examined the ultrastructure of R. elongata sp. nov., which revealed that it is more complicated than the previously described R. libera. Our data show that either the endobiotic lifestyle of some Rhizomastix species has arisen independently from other endobiotic archamoebae, or the free-living members of this genus represent a secondary switch from the endobiotic lifestyle.
References provided by Crossref.org
Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans
GENBANK
GU921236, KP343610, KP343638
