Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed.

Brno University of Technology CEITEC Central European Institute of Technology Technicka 3058 10 Brno 61600 Czech Republic

Brno University of Technology Institute of Physical Engineering Faculty of Mechanical Engineering Technicka 2896 2 Brno 61600 Czech Republic

Brno University of Technology Institute of Physical Engineering Faculty of Mechanical Engineering Technicka 2896 2 Brno 61600 Czech RepublicbBrno University of Technology CEITEC Central European Institute of Technology Technicka 3058 10 Brno 61600

University of California San Diego 9500 Gilman Drive La Jolla California 92093 United States

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