Broad-range TRP channel inhibitors (2-APB, flufenamic acid, SKF-96365) affect differently contraction of resistance and conduit femoral arteries of rat
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26384458
DOI
10.1016/j.ejphar.2015.09.014
PII: S0014-2999(15)30246-6
Knihovny.cz E-zdroje
- Klíčová slova
- 2-APB, Femoral artery, Flufenamic acid, SKF-96365, TRP channel, Vascular contraction,
- MeSH
- arteria femoralis účinky léků fyziologie MeSH
- imidazoly farmakologie MeSH
- kationtové kanály TRP antagonisté a inhibitory fyziologie MeSH
- krysa rodu Rattus MeSH
- kyselina flufenamová farmakologie MeSH
- orgánové kultury - kultivační techniky MeSH
- potkani Wistar MeSH
- sloučeniny boru farmakologie MeSH
- svaly hladké cévní účinky léků fyziologie MeSH
- vazokonstrikce účinky léků fyziologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole MeSH Prohlížeč
- 2-aminoethoxydiphenyl borate MeSH Prohlížeč
- imidazoly MeSH
- kationtové kanály TRP MeSH
- kyselina flufenamová MeSH
- sloučeniny boru MeSH
Transient receptor potential (TRP) channels are proposed to contribute to membrane depolarization and Ca2+ influx into vascular smooth muscle (VSM) cells. Our aim was to study the effects of widely used broad-range TRP channel inhibitors--2-aminoethoxydiphenyl borate (2-APB), flufenamic acid (FFA) and SKF-96365--on the contraction of freshly isolated small and large arteries. Endothelium-denuded resistance (≈250 µm) and conduit (≈1000 µm) femoral arteries were isolated from adult Wistar rats and mounted in wire myograph. The effects of the above mentioned TRP channel inhibitors and voltage-dependent calcium channel inhibitor nifedipine were studied on arterial contractions induced by phenylephrine, U-46619 or K+. Phenylephrine-induced contractions were also studied in the absence of extracellular Na+. mRNA expression of particular canonical and melastatin TRP channel subunits in femoral vascular bed was determined. TRP channel inhibitors attenuated K+-induced contraction less than nifedipine. Phenylephrine-induced contraction was more influenced by 2-APB in resistance arteries, while FFA completely prevented U-46619-induced contraction in both sizes of arteries. The absence of extracellular Na+ prevented the inhibitory effects of 2-APB, but not those of FFA. The observed effects of broad-range TRP channel inhibitors, which were dependent on the size of the artery, confirmed the involvement of TRP channels in agonist-induced contractions. The inhibitory effects of 2-APB (but not those of FFA or SKF-96365) were dependent on the presence of extracellular Na+.
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