Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications
Language English Country United States Media print-electronic
Document type Journal Article
- Keywords
- biosensor, electrochemistry, glycidyl methacrylate, horseradish peroxidase, nanospheres, thionine,
- MeSH
- Biosensing Techniques methods MeSH
- Electrochemistry MeSH
- Electrons MeSH
- Enzymes, Immobilized chemistry MeSH
- Phenothiazines chemistry MeSH
- Hydrolysis MeSH
- Horseradish Peroxidase chemistry MeSH
- Polymethacrylic Acids chemistry MeSH
- Oxygen chemistry MeSH
- Microscopy, Electron, Scanning MeSH
- Nanospheres chemistry MeSH
- Nanotechnology methods MeSH
- Polymerization MeSH
- Polystyrenes chemistry MeSH
- Antibodies chemistry MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Particle Size MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Enzymes, Immobilized MeSH
- Phenothiazines MeSH
- Horseradish Peroxidase MeSH
- Polymethacrylic Acids MeSH
- Oxygen MeSH
- polyglycidyl methacrylate MeSH Browser
- Polystyrenes MeSH
- Antibodies MeSH
- thionine MeSH Browser
Monodisperse poly(glycidyl methacrylate) (PGMA) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. The effects of various reaction parameters on the particle properties were investigated. The particle size was controlled in the range of 350-420 nm. To introduce carboxyl groups, the PGMA nanospheres were hydrolyzed and oxidized with KMnO4. Subsequently, the enzyme horseradish peroxidase (HRP) and the electron mediator thionine were covalently attached to the PGMA nanospheres to obtain an antibody indicator suitable for enzyme-based electrochemical immunosensors. Combined HRP and thionine binding to the nanospheres had beneficial effects for the labeling efficiency and at the same time prevented the formation of soluble electron mediators.
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