Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26479119
DOI
10.1021/acsami.5b08469
Knihovny.cz E-zdroje
- Klíčová slova
- biosensor, electrochemistry, glycidyl methacrylate, horseradish peroxidase, nanospheres, thionine,
- MeSH
- biosenzitivní techniky metody MeSH
- elektrochemie MeSH
- elektrony MeSH
- enzymy imobilizované chemie MeSH
- fenothiaziny chemie MeSH
- hydrolýza MeSH
- křenová peroxidasa chemie MeSH
- kyseliny polymethakrylové chemie MeSH
- kyslík chemie MeSH
- mikroskopie elektronová rastrovací MeSH
- nanokuličky chemie MeSH
- nanotechnologie metody MeSH
- polymerizace MeSH
- polystyreny chemie MeSH
- protilátky chemie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- enzymy imobilizované MeSH
- fenothiaziny MeSH
- křenová peroxidasa MeSH
- kyseliny polymethakrylové MeSH
- kyslík MeSH
- polyglycidyl methacrylate MeSH Prohlížeč
- polystyreny MeSH
- protilátky MeSH
- thionine MeSH Prohlížeč
Monodisperse poly(glycidyl methacrylate) (PGMA) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. The effects of various reaction parameters on the particle properties were investigated. The particle size was controlled in the range of 350-420 nm. To introduce carboxyl groups, the PGMA nanospheres were hydrolyzed and oxidized with KMnO4. Subsequently, the enzyme horseradish peroxidase (HRP) and the electron mediator thionine were covalently attached to the PGMA nanospheres to obtain an antibody indicator suitable for enzyme-based electrochemical immunosensors. Combined HRP and thionine binding to the nanospheres had beneficial effects for the labeling efficiency and at the same time prevented the formation of soluble electron mediators.
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