Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
R01 HL108882
NHLBI NIH HHS - United States
T32 GM007445
NIGMS NIH HHS - United States
R01HL108882
NHLBI NIH HHS - United States
PubMed
26482708
PubMed Central
PMC4690203
DOI
10.1016/j.bbabio.2015.10.004
PII: S0005-2728(15)00211-X
Knihovny.cz E-zdroje
- Klíčová slova
- Mitochondria, Morphology, Phosphatidylglycerol, Respiration, Yeast,
- MeSH
- fosfatidylglyceroly metabolismus MeSH
- fosfolipasy fyziologie MeSH
- kardiolipiny biosyntéza MeSH
- mitochondrie metabolismus ultrastruktura MeSH
- respirační komplex IV metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- fosfatidylglyceroly MeSH
- fosfolipasy MeSH
- kardiolipiny MeSH
- respirační komplex IV MeSH
In yeast, phosphatidylglycerol (PG) is a minor phospholipid under standard conditions; it can be utilized for cardiolipin (CL) biosynthesis by CL synthase, Crd1p, or alternatively degraded by the phospholipase Pgc1p. The Saccharomyces cerevisiae deletion mutants crd1Δ and pgc1Δ both accumulate PG. Based on analyses of the phospholipid content of pgc1Δ and crd1Δ yeast, we revealed that in yeast mitochondria, two separate pools of PG are present, which differ in their fatty acid composition and accessibility for Pgc1p-catalyzed degradation. In contrast to CL-deficient crd1Δ yeast, the pgc1Δ mutant contains normal levels of CL. This makes the pgc1Δ strain a suitable model to study the effect of accumulation of PG per se. Using fluorescence microscopy, we show that accumulation of PG with normal levels of CL resulted in increased fragmentation of mitochondria, while in the absence of CL, accumulation of PG led to the formation of large mitochondrial sheets. We also show that pgc1Δ mitochondria exhibited increased respiration rates due to increased activity of cytochrome c oxidase. Taken together, our results indicate that not only a lack of anionic phospholipids, but also excess PG, or unbalanced ratios of anionic phospholipids in mitochondrial membranes, have harmful consequences on mitochondrial morphology and function.
Department of Biochemistry Faculty of Natural Sciences Comenius University Bratislava Slovakia
Department of Physiology School of Medicine Johns Hopkins University Baltimore MD USA
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